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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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A dansyl-based fluorescent probe for sensing Cu2+ in aqueous solution.

Yu Liu1, Bing Jiang1, Linlin Zhao1

  • 1School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|June 4, 2021
PubMed
Summary
This summary is machine-generated.

A novel fluorescent probe, D-GT, effectively detects copper ions (Cu2+) in aqueous solutions. This probe shows high sensitivity and potential for real-world applications, including in living cells.

Keywords:
CoordinationCu(2+)DansylFluorescent probeFluorescent quenching

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Copper ions (Cu2+) play crucial roles in biological processes but elevated levels can be toxic.
  • Development of sensitive and selective fluorescent probes is essential for monitoring Cu2+ in various environments.

Purpose of the Study:

  • To design and synthesize a novel fluorescent probe, D-GT, for the detection of Cu2+.
  • To evaluate the probe's performance in aqueous solutions and complex biological samples.

Main Methods:

  • Synthesis of a glycyl-L-tyrosine-modified dansyl derivative (D-GT).
  • Spectroscopic analysis (fluorescence quenching) to determine Cu2+ interaction.
  • Determination of binding stoichiometry and association constant.
  • Detection limit assessment in buffer solutions, drinking water, and living cells.

Main Results:

  • D-GT demonstrated sensitive fluorescence quenching upon binding with Cu2+.
  • The probe exhibited a wide pH working range (6-12) and a 1:1 binding stoichiometry.
  • An association constant of 6.47 × 104 M-1 was determined.
  • A low detection limit of 0.69 μM for Cu2+ was achieved in HEPES buffer.

Conclusions:

  • The D-GT probe offers excellent sensitivity and selectivity for Cu2+ detection.
  • The probe's effectiveness in real samples and living cells highlights its potential for environmental and biological monitoring.
  • D-GT represents a promising tool for studying copper ion dynamics in complex systems.