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Updated: Aug 16, 2026

Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
Published on: September 17, 2008
Proteolytic specificity of chicken cathepsin L on bovine beta-casein
1S.R.V., I.N.R.A. de Theix, Ceyrat, France.
Abstract:
The proteolytic specificity of chicken cathepsin L was studied using bovine beta-casein as substrate. The peptide mixtures obtained after various times of hydrolysis were separated by RP-HPLC and ten peptides were identified. Chicken cathepsin L accepts proline residues in all positions except P'1. Looking at the amino acid residues on the amino side of the scissile bond we found three times the Tyr-Pro pair at P'1-P'2 positions and that the S'1 subsite can interact with modified amino acids such as phosphoserine.
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