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Cutibacterium spp. isolated from orthopaedic implant-associated infection: A not-so-slowly growing organism

Llanos Salar-Vidal1, Marta Martin-Garcia1, Alvaro Auñón1

  • 1Departments of Clinical Microbiology and *Orthopaedic Surgery, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain.

Enfermedades Infecciosas Y Microbiologia Clinica (English Ed.)
|June 5, 2021
PubMed
Abstract

Insights

Most clinically significant Cutibacterium spp. infections are detected within 7 days. Prolonged incubation up to 14 days may be needed for other organisms, but not typically for Cutibacterium spp. in orthopedic samples.

Area of Science:

  • Microbiology
  • Orthopedic Surgery

Background:

  • Microbiological diagnosis of Cutibacterium spp. infections traditionally requires up to 14 days of incubation.
  • Cutibacterium spp. are increasingly recognized pathogens in orthopedic device-related infections.

Purpose of the Study:

  • To evaluate the incubation time required for the detection of Cutibacterium spp. in orthopedic samples.
  • To determine if prolonged incubation beyond 7 days is necessary for clinically significant Cutibacterium spp. detection.

Main Methods:

  • Retrospective analysis of 119 orthopedic samples (implants, biopsies, synovial fluid) over 10 years.
  • Cultures were incubated, and the number of days to positive growth for Cutibacterium spp. was recorded.
  • Atkins's criteria were used for interpreting isolates.

Main Results:

  • The median incubation time for detecting clinically significant Cutibacterium spp. isolates was 4 days.
  • No clinically significant Cutibacterium spp. isolates were detected after 8 days of incubation.
  • Contaminant isolates also showed similar detection times.

Conclusions:

  • Clinically significant Cutibacterium spp. are typically detected within the first 7 days of incubation.
  • Prolonged incubation up to 14 days is generally not required for Cutibacterium spp. in orthopedic samples.
  • Standard incubation periods may be sufficient, reducing laboratory workload and turnaround time.
Keywords:
Cutibacterium spp.Diagnóstico infección articularIncubation timeInfección de prótesis articularInfection diagnosisProsthetic jointProsthetic joint infectionPrótesis articularTiempo de incubación

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