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The ESCRT-III complex contributes to macromitophagy in yeast.

Zulin Wu1, Haiqian Xu1, Junze Liu1

  • 1College of Life Sciences, Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, China.

Traffic (Copenhagen, Denmark)
|June 5, 2021
PubMed
Summary

The endosomal sorting complex required for transport III (ESCRT-III) machinery regulates macromitophagy, a process for removing damaged mitochondria. This study reveals ESCRT-III

Keywords:
Atg11Atg32ESCRTVps4macromitophagymicromitophagymitophagosomemitophagy, Snf7

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Mitochondria are vital for cellular energy and homeostasis.
  • Mitophagy, a selective form of autophagy, removes damaged mitochondria.
  • Macromitophagy and micromitophagy are distinct selective mitophagy pathways.

Purpose of the Study:

  • To investigate the role of the ESCRT-III complex in yeast macromitophagy.
  • To determine if ESCRT-III regulates mitophagy induced by different conditions.
  • To elucidate the molecular mechanism of ESCRT-III in mitophagy.

Main Methods:

  • Monitoring mitochondrial marker Om45-GFP.
  • Assessing degradation of Om45-GFP to vacuoles.
  • Utilizing protease-protection assays.
  • Employing glutathione-S-transferase (GST) pulldown and bimolecular fluorescence complementation (BiFC) assays.
  • Analyzing mitophagy receptor Atg32 and autophagosome marker Atg8.

Main Results:

  • Loss of ESCRT-III subunits Snf7 or Vps4 suppresses Om45-GFP delivery and degradation.
  • Om45 and Atg32 accumulate on autophagosomes (mitophagosomes) in ESCRT mutants.
  • ESCRT-III components Snf7 and Vps4 are involved in mitophagosome closure.
  • Snf7 interacts with Atg11 on the mitochondrial reticulum.

Conclusions:

  • ESCRT-III machinery mediates nitrogen starvation-induced macromitophagy in yeast.
  • The ESCRT-III complex plays a novel role in regulating macromitophagy.
  • Interaction between Snf7 and Atg11 is crucial for recruiting ESCRT-III to mitophagosomes.