Anion species-triggered antibody separation system utilizing a thermo-responsive polymer column under optimized...

Area of Science:

• Biotechnology
• Polymer Chemistry
• Biochemistry

Background:

• Antibody production faces challenges in purification, with conventional methods being costly and causing aggregation.
• There is a need for alternative, efficient antibody purification strategies.

Purpose of the Study:

• To develop a constant temperature antibody purification system using a thermo-responsive polymer column.
• To enable efficient separation of therapeutic monoclonal antibodies from contaminants.

Main Methods:

• Synthesized thermo-responsive hydrogel-modified silica beads via graft-from approach.
• Developed a purification system utilizing anion species switching in eluents at constant temperature.
• Tested elution behavior of antibodies with citrate, phosphate, and chloride anions.

Main Results:

• The developed column demonstrated switchable antibody retention behavior at constant temperature and salt concentration.
• Antibody adsorption was achieved using NaCl eluent, and desorption using citric acid buffer eluent.
• Successful separation of therapeutic monoclonal antibodies (rituximab and trastuzumab) from contaminants was achieved.

Conclusions:

• The thermo-responsive polymer column provides an effective and alternative method for antibody purification.
• This system offers advantages over conventional methods by maintaining constant salt concentration and pH, and reducing aggregation.
• The developed purification system shows potential for therapeutic antibody manufacturing.