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Developing Rhodobacter sphaeroides for cathodic biopolymer production.

Ferdinand Schmid1, Julia Novion Ducassou2, Yohann Couté2

  • 1Institute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Karlsruhe Germany.

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Rhodobacter sphaeroides shows promise for photoautotrophic production. This bacterium efficiently forms biofilms on cathodes in bioelectrochemical systems, enabling poly(3-hydroxybutyrate) accumulation.

Keywords:
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Area of Science:

  • Microbial electrochemistry
  • Synthetic biology
  • Biotechnology

Background:

  • Photoautotrophic production processes offer sustainable manufacturing routes.
  • Identifying robust microbial strains for bioelectrochemical systems (BES) is crucial for process efficiency.
  • Rhodobacter sphaeroides is explored for its potential in cathodic applications.

Purpose of the Study:

  • To evaluate Rhodobacter sphaeroides as a cathodic production strain in photoautotrophic BES.
  • To investigate the impact of genetic modification and selection pressure on cathodic performance.
  • To characterize biofilm formation and poly(3-hydroxybutyrate) (PHB) accumulation.

Main Methods:

  • Stable cultivation of Rhodobacter sphaeroides in a BES using a poised cathode as an electron donor.
  • Introduction of a plasmid vector and antibiotic selection.
  • Quantitative proteomic analysis and optical coherence tomography (OCT) for biofilm characterization.
  • Cultivation under nitrogen-limited conditions for PHB accumulation.

Main Results:

  • Stable cultivation and biofilm formation on the cathode were achieved.
  • Plasmid introduction and antibiotic pressure significantly enhanced biofilm formation and electrochemical properties.
  • Proteomic analysis revealed key molecular adaptations for cathodic biofilm growth.
  • OCT confirmed robust biofilm architecture and kinetics.
  • Nitrogen limitation induced significant poly(3-hydroxybutyrate) accumulation.

Conclusions:

  • Rhodobacter sphaeroides is a promising cathodic production strain for photoautotrophic processes.
  • Genetic manipulation and selection pressure optimize its performance in BES.
  • The strain demonstrates potential for industrial-scale photoautotrophic poly(3-hydroxybutyrate) production.