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Long non-coding RNA XIST regulates ovarian cancer progression via modulating miR-335/BCL2L2 axis

Qingjuan Meng1, Ningning Wang2, Guanglan Duan3

  • 1Medical Examination Center, The Third Hospital of Jinan, Jinan, 250132, China.

World Journal of Surgical Oncology
|June 6, 2021
PubMed
Abstract

Insights

XIST long non-coding RNA (lncRNA) promotes ovarian cancer progression by regulating the miR-335/BCL2L2 axis. Inhibiting XIST suppressed tumor growth and metastasis, identifying XIST as a potential therapeutic target for ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • X inactivation-specific transcript (XIST) is a long non-coding RNA implicated in various cancers.
  • The precise role of XIST in ovarian cancer progression remains unclear.
  • This study investigates XIST function in ovarian cancer cells and tissues.

Purpose of the Study:

  • To elucidate the function and molecular mechanism of XIST in ovarian cancer.
  • To determine if XIST influences ovarian cancer cell proliferation, migration, and invasion.
  • To explore the involvement of the miR-335/BCL2L2 axis in XIST-mediated ovarian cancer progression.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to measure miR-335 and BCL2L2 expression.
  • Cell proliferation, migration, and invasion assays (MTT, Transwell).
  • Western blot for BCL2L2 protein levels and luciferase reporter assays to confirm molecular interactions.

Main Results:

  • Inhibition of XIST significantly reduced ovarian cancer cell proliferation, invasion, and migration.
  • The miR-335/BCL2L2 axis was identified as a key mediator of XIST's effects.
  • XIST regulates tumor progression through modulation of the miR-335/BCL2L2 pathway.

Conclusions:

  • XIST acts as a carcinogenic lncRNA in ovarian cancer.
  • XIST promotes ovarian cancer development by regulating miR-335.
  • XIST represents a promising therapeutic target for ovarian cancer treatment.
Keywords:
BCL2L2Ovarian cancerXISTmiR-335

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