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Related Experiment Videos

Electron microscopy of plasticity in rat olfactory cortex.

L E Westrum1

  • 1Department of Neurological Surgery, University of Washington, Seattle 98195.

Brain Research
|July 1, 1988
PubMed
Summary

Young rats show significant synaptic reorganization in the olfactory cortex after olfactory bulb removal. Critical developmental periods (postnatal days 9-13) are key for this age-dependent plasticity.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Electron Microscopy

Background:

  • Olfactory cortex (OC) undergoes age-dependent reorganization of afferents.
  • Deafferentation by olfactory bulb (OB) removal triggers this reorganization.

Purpose of the Study:

  • To investigate the ultrastructural basis of age-dependent afferent reorganization in the rat OC post-deafferentation using electron microscopy (EM).
  • To identify critical developmental periods for synaptic plasticity in the OC.

Main Methods:

  • Double-lesion technique: primary OB lesion at various postnatal (PN) ages (0-100), followed by a secondary OC lesion.
  • EM analysis of dendritic layers (Ia and Ib) rostral to the OC lesion.
  • Examination of degenerating synaptic terminals and glial debris.

Main Results:

  • In adults with early OB lesions (PN 0-9), OC lesions cause degenerating terminals throughout Ia and Ib.
  • Degenerating terminals in Ia decrease significantly by PN 13 and are rare/absent in older ages (PN 30-100).
  • Glial debris increases with age of OB lesion; transsynaptic alterations observed, especially in older groups.

Conclusions:

  • Postnatal days 9-13 represent critical ages for developmental plasticity in the rat OC.
  • Synapses are involved in this plasticity, potentially through reinnervation or persistence of existing synapses.
  • EM findings support earlier light microscopy observations and clarify the nature of degeneration particles.

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