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Updated: Nov 3, 2025

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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Chitosan-based nanofibers for enzyme immobilization.

Eduardo Silveira Ribeiro1, Bruna Silva de Farias2, Tito Roberto Sant'Anna Cadaval Junior2

  • 1Biotechnology Unit, Technology Development Center, Federal University of Pelotas, Campus Capão do Leão, Capão do Leão, Brazil.

International Journal of Biological Macromolecules
|June 6, 2021
PubMed
Summary

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Chitosan nanofibers enhance enzyme stability for industrial use. This review examines immobilization methods, highlighting chitosan

Area of Science:

  • Biotechnology and Materials Science

Background:

  • Soluble enzyme instability limits industrial applications.
  • Enzyme immobilization is a key strategy to enhance enzyme stability and reusability.
  • Chitosan-based nanofibers offer unique properties for effective enzyme immobilization.

Purpose of the Study:

  • To review advantages and disadvantages of enzyme immobilization methods.
  • To explore the potential of chitosan-based nanofibers for enzyme immobilization.
  • To discuss challenges and future prospects in this field.

Main Methods:

  • Review of adsorption, cross-linking, and entrapment techniques for enzyme immobilization.
  • Analysis of chitosan-based nanofibers' properties relevant to immobilization.
  • Discussion of current challenges and future directions.
Keywords:
AdsorptionCross–linkingElectrospinningEntrapment

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Main Results:

  • Chitosan nanofibers possess properties like large surface area, porosity, and functional groups beneficial for enzyme immobilization.
  • Existing immobilization methods (adsorption, cross-linking, entrapment) have unresolved questions regarding mechanisms and limitations.
  • Chitosan nanofibers show promise for improving enzyme catalytic activity and operational lifetime.

Conclusions:

  • Chitosan-based nanofibers present a promising platform for overcoming limitations in soluble enzyme applications.
  • Further research is needed to address challenges in adsorption mechanisms, cross-linking effects, and diffusion limitations.
  • Optimizing chitosan nanofiber-based enzyme immobilization can maximize enzyme performance in industrial settings.