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Updated: Nov 3, 2025

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Published on: March 27, 2018

A simplified method for separating renal MPCs using SLAMF9.

Joseph A Mikulin¹, Briana L Bates¹, Timothy J Wilson¹

¹Department of Microbiology, Miami University, Oxford, Ohio, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|June 6, 2021

Summary

This summary is machine-generated.

SLAMF9 is a novel marker for identifying kidney-resident mononuclear phagocytes (MPCs). This discovery simplifies the isolation of these crucial immune cells for further research.

Keywords:

SLAMF9 dendritic cells kidney mononuclear phagocytes

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Area of Science:

Immunology
Cell Biology
Renal Science

Background:

Mononuclear phagocytes (MPCs) are vital for tissue homeostasis and infection resistance.
Their diverse phenotypes complicate functional characterization within tissues, often requiring multiple surface markers.

Purpose of the Study:

To identify a specific marker for kidney-resident MPCs to simplify their isolation and study.
To investigate the utility of SLAMF9 as a marker for renal MPC populations.

Main Methods:

Multi-parametric flow cytometry was used to analyze SLAMF9 expression in renal mononuclear phagocyte populations.
SLAMF9 expression was assessed for its ability to identify and sort specific MPC subsets from disaggregated mouse kidney tissue.

Main Results:

SLAMF9 expression specifically identifies kidney-resident CD45+ CD11c+ MHC-II+ cells, corresponding to dendritic cell progenitor-derived MPCs.
High SLAMF9 expression enabled efficient sorting of these cells using a standard cell sorter.
SLAMF9 facilitated further subdivision of MPCs based on CD11b and CD14 expression, distinguishing cDC1 and other MPC subsets.

Conclusions:

SLAMF9 is a reliable and specific marker for identifying and isolating kidney-resident MPCs.
This simplifies the study of these cells, eliminating the need for complex antibody panels or reporter mice.
Facilitates ex vivo studies of renal MPCs for improved understanding of kidney function and disease.