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Published on: April 27, 2011
Temporal phylogeny and molecular characterization of echovirus 30 associated with aseptic meningitis outbreaks in
Xiaoling Tian1, Zhenzhi Han2,3, Yulong He4
1Inner Mongolia Center for Disease Control and Prevention, Huhhot, 010031, People's Republic of China.
Background:
An outbreak of aseptic meningitis occurred from June to August 2016, in Inner Mongolia Autonomous Region, China.
Methods:
To determine its epidemiological characteristics, etiologic agent, and possible origin, specimens were collected for virus isolation and identification, followed by molecular epidemiological analysis.
Results:
A total of 363 patients were clinically diagnosed from June 1st to August 31st 2016, and most cases (63.1%, n = 229) were identified between June 22nd and July 17th, with children aged 6 to 12 years constituting the highest percentage (68.9%, n = 250). All viral isolates from this study belonged to genotype C of echovirus 30 (E30), which dominated transmission in China. To date, two E30 transmission lineages have been identified in China, of which Lineage 2 was predominant. We observed fluctuant progress of E30 genetic diversity, with Lineage 2 contributing to increased genetic diversity after 2002, whereas Lineage 1 was significant for the genetic diversity of E30 before 2002.
Conclusions:
We identified the epidemiological and etiological causes of an aseptic meningitis outbreak in Inner Mongolia in 2016, and found that Lineage 2 played an important role in recent outbreaks. Moreover, we found that Gansu province could play an important role in E30 spread and might be a possible origin site. Furthermore, Fujian, Shandong, Taiwan, and Zhejiang provinces also demonstrated significant involvement in E30 evolution and persistence over time in China.
Insights
An aseptic meningitis outbreak in Inner Mongolia was caused by echovirus 30 (E30) genotype C. Lineage 2 of E30 was predominant in recent outbreaks and contributed to genetic diversity.
Area of Science:
- Virology
- Epidemiology
- Molecular Biology
Background:
- An outbreak of aseptic meningitis occurred in Inner Mongolia, China, between June and August 2016.
- This outbreak primarily affected children aged 6 to 12 years.
Purpose of the Study:
- To investigate the epidemiological characteristics of the aseptic meningitis outbreak.
- To identify the etiologic agent and its possible origin.
- To conduct molecular epidemiological analysis of the causative agent.
Main Methods:
- Collected specimens for virus isolation and identification.
- Performed molecular epidemiological analysis on viral isolates.
- Analyzed epidemiological data from 363 diagnosed patients.
Main Results:
- All viral isolates were identified as echovirus 30 (E30) genotype C.
- The outbreak peaked between June 22nd and July 17th, 2016.
- Echovirus 30 Lineage 2 was predominant in China and contributed to increased genetic diversity after 2002.
Conclusions:
- Echovirus 30 Lineage 2 plays a significant role in recent aseptic meningitis outbreaks in China.
- Gansu province is identified as a potential origin site for E30 spread.
- Fujian, Shandong, Taiwan, and Zhejiang provinces are implicated in the evolution and persistence of E30 in China.
