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Updated: Nov 3, 2025

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
"CLipP"ing on lipids to generate antibacterial lipopeptides
Victor Yim1,2, Iman Kavianinia1,3,2, Melanie K Knottenbelt4
1School of Biological Sciences, University of Auckland 3A Symonds Street Auckland 1010 New Zealand.
Abstract:
We herein report the synthesis and biological and computational evaluation of 12 linear analogues of the cyclic lipopeptide battacin, enabled by Cysteine Lipidation on a Peptide or Amino Acid (CLipPA) technology. Several of the novel "CLipP"ed lipopeptides exhibited low micromolar MICs and MBCs against both Gram-negative and Gram-positive bacteria. The mechanism of action was then simulated with the MIC data using computational methods.
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