The dark side of disulfide-based dynamic combinatorial chemistry
Mélissa Dumartin1, Jean Septavaux1,2, Marion Donnier-Maréchal1
1Univ. Lyon, Univ. Lyon 1, CNRS, INSA, CPE, ICBMS F-69622 Lyon France florent.perret@univ-lyon1.fr laurent.vial@univ-lyon1.fr julien.leclaire@univ-lyon1.fr.
Chemical Science
|June 7, 2021
Summary
Dynamic combinatorial chemistry using disulfide bonds is key for creating artificial receptors. Comprehensive analysis beyond HPLC-MS is crucial for understanding library composition and function in molecular recognition.
Area of Science:
- Chemistry
- Biochemistry
- Molecular Recognition
Background:
- Disulfide-based dynamic combinatorial chemistry (DCC) is widely used for developing artificial receptors.
- HPLC-MS is commonly employed to analyze DCC libraries and identify potential binders for biomolecules.
Purpose of the Study:
- To re-investigate DCC libraries generated from a 2,5-dicarboxy-1,4-dithiophenol building block in water.
- To demonstrate the necessity of multiple analytical tools for comprehensive library characterization.
Main Methods:
- Generation of dynamic combinatorial libraries using a specific building block in aqueous media.
- Application of multiple analytical techniques for library analysis.
Main Results:
- Comprehensive characterization of library members in terms of size and stereochemistry.
- Detailed assessment of binding affinity and selectivity for target molecules.
- Deeper understanding of the phenomena governing dynamic combinatorial systems.
Conclusions:
- Multiple analytical tools are essential for a thorough description of DCC libraries.
- Accurate characterization is vital for understanding molecular recognition and receptor design.
- This study highlights limitations of relying solely on HPLC-MS for DCC analysis.
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