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Comparison of the Japan Society of Clinical Chemistry reference method and CDC method for HDL and LDL cholesterol
Yuzo Kayamori1, Masakazu Nakamura2, Koji Kishi3
1Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka City, Fukuoka, 812-8582, Japan.
Insights
The Japan Society of Clinical Chemistry (JSCC) method for measuring high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) shows excellent agreement with the traditional Centers for Disease Control and Prevention (CDC)-beta-quantification (BQ) method.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Cardiovascular Diagnostics
Background:
- The Japan Society of Clinical Chemistry (JSCC) proposed a reference method in 2009 for serum HDL-C and LDL-C measurement.
- This automated method involves cholesterol esterase-cholesterol dehydrogenase after ultracentrifugation and precipitation.
Purpose of the Study:
- To compare LDL-C and HDL-C levels measured by the JSCC method against the traditional CDC-beta-quantification (BQ) method.
- To validate the JSCC method's accuracy in diverse patient populations.
Main Methods:
- LDL-C and HDL-C levels were measured in 173 subjects (47 non-diseased, 126 diseased) with triglyceride levels below 11.29 mmol/L.
- Both the JSCC and CDC-BQ methods were employed for comparative analysis.
Main Results:
- For LDL-C, the correlation equation was y = 0.991x + 0.009 mmol/L (r=0.999).
- For HDL-C, the correlation equation was y = 0.988x + 0.041 mmol/L (r=0.999).
- Both methods demonstrated high correlation coefficients (r=0.999) and low standard errors of the estimate (Sy/x).
Conclusions:
- The JSCC method demonstrates strong agreement with the CDC-BQ method for both LDL-C and HDL-C measurements.
- This agreement holds true for both non-diseased and diseased subjects, including those with dyslipidemia.
Objectives:
In 2009, the Japan Society of Clinical Chemistry (JSCC) recommended a reference method for the measurement of serum high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels. This automated method uses cholesterol esterase-cholesterol dehydrogenase to measure cholesterol levels in fractions obtained after ultracentrifugation and dextran sulfate/magnesium chloride precipitation. In the present study, using fresh samples, we compared the LDL-C and HDL-C levels measured using this method with those measured using the traditional Centers for Disease Control and Prevention (CDC)-beta-quantification (BQ) method.
Design:
and methods: Using both the JSCC and CDC-BQ methods, LDL-C/HDL-C levels were measured in 47 non-diseased and 126 diseased subjects, whose triglyceride levels were lower than 11.29 mmol/L (1000 mg/dL).
Results:
For LDL-C, the equation of the line representing the correlation between the two methods was y = 0.991x + 0.009 mmol/L; r = 0.999; and Sy/x = 0.025 mmol/L, where x is the mean LDL-C level measured using the CDC-BQ method. Similarly, for HDL-C, the equation of the line representing the correlation between the two methods was y = 0.988x + 0.041 mmol/L, r = 0.999, and Sy/x = 0.019 mmol/L, where x is the mean HDL-C level measured using the CDC-BQ method.
Conclusions:
The JSCC method agreed with the CDC-BQ method in cases of both non-diseased and diseased subjects, including those with dyslipidemia.
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