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Updated: Nov 2, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
Integrating Somatic and Germline Next-Generation Sequencing Into Routine Clinical Oncology Practice
J Kevin Hicks1,2, Rachel Howard3, Phillip Reisman3
1Department of Individualized Cancer Management, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL.
Next-generation sequencing (NGS) integrates genetic insights into cancer care for personalized treatment. This involves optimizing test ordering, reporting, interpretation, and electronic health record integration for better patient outcomes.
Area of Science:
- Oncology
- Genomics
- Clinical Informatics
Background:
- Next-generation sequencing (NGS) is increasingly used in oncology for hereditary cancer risk, prognosis, and treatment.
- Genetic variations in cancer and inherited genomes offer critical clinical information.
Purpose of the Study:
- To outline the clinical perspective of integrating NGS results into patient care for therapeutic decision-making.
- To address key considerations for operationalizing NGS testing and applying results.
Main Methods:
- Focus on five key considerations: test ordering/workflow, reporting/curation/storage, clinical consultation, electronic health record integration, and education.
- Emphasis on informatics tools for NGS test utilization and clinical decision support.
Main Results:
- Informatics tools are crucial for NGS test ordering and referencing results for therapy.
- Clinical decision support within electronic health records aids NGS utilization and targeted therapy identification, including clinical trials.
Conclusions:
- Operationalizing NGS-supported, evidence-based patient care requires addressing informatics and clinical data standards.
- Solutions for overcoming challenges in integrating NGS into routine oncology practice are discussed.
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