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Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag

Christine K Rourke1, Darline Murat1, Tyler J Hansen2

  • 1Department of Biological Sciences, University of Delaware, Newark, Delaware 19716.

Micropublication Biology
|June 7, 2021
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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