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Characterizing brain metabolic function ex vivo with acute mouse slice punches.
Guoyuan Qi1, Yashi Mi1, Fei Yin1,2,3
1Center for Innovation in Brain Science, University of Arizona Health Sciences, University of Arizona, 1230 N Cherry Ave, Tucson, AZ 85724, USA.
STAR Protocols
|June 7, 2021
Summary
This study introduces a new protocol for profiling brain metabolic function in mouse slices ex vivo. It helps understand energy metabolism shifts in neurological diseases.
Area of Science:
- Neuroscience
- Metabolic research
- Biochemistry
Background:
- Mitochondrial dysfunction and metabolic reprogramming are linked to neurological disorders.
- Understanding brain energy metabolism is crucial for disease research.
Purpose of the Study:
- To present a protocol for complex profiling of brain metabolic function using acute mouse brain slices ex vivo.
- To enable the study of metabolic shifts in neurological conditions.
Main Methods:
- Utilizing acute mouse brain slices ex vivo.
- Employing differential metabolic conditions, substrates, and inhibitors.
- Complex profiling of brain metabolic function.
Main Results:
- The protocol allows for broad application in studying metabolic shifts.
- It can be used to analyze responses to genetic manipulations, pathological insults, or therapeutic interventions.
Conclusions:
- This protocol can advance the understanding of energy metabolism's role in brain function and diseases.
- It provides a tool for investigating metabolic reprogramming in neurological disorders.
