Related Experiment Video
Updated: Nov 2, 2025

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Published on: May 19, 2023
Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis
Francisco X Carvalho1, Bárbara Guerra-Carvalho1, Ivana Jarak2
1Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal.
Abstract:
Mitochondria play a central role in metabolic reprograming that occurs in numerous disease conditions. A precise evaluation of the extent of mitochondrial involvement in the metabolic alterations is essential for a better definition of metabolically based therapeutic strategies. In this chapter, some simple protocols are presented, using carbon 13 tracers and nuclear magnetic resonance isotopomer analysis, for the evaluation of mitochondrial contributions to intermediary metabolism and the metabolic effects of the implementation of some mitochondrial regulatory mechanisms.

