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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Published on: May 22, 2021

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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling.

Shreeya Kedia1, Sarah L Erickson2, Guang Yang3

  • 1Department of Biochemistry and Molecular Biology; Cumming School of Medicine, University of Calgary; Alberta Children's Hospital Research Institute, University of Calgary.

Journal of Visualized Experiments : Jove
|June 7, 2021
PubMed
Summary
This summary is machine-generated.

This study details a new method for analyzing mRNA translation in developing mouse brains. This technique helps understand how neural stem cell fate is controlled during brain development.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Proper mammalian brain development requires balancing neural stem cell proliferation and differentiation.
  • Gene expression, regulated at transcriptional, post-transcriptional, and translational levels, controls this balance.
  • Translational regulation plays a critical role in neural stem cell fate decisions.

Purpose of the Study:

  • To establish an in-house polysome profiling pipeline.
  • To assess mRNA translational efficiency in cells from the developing mouse cerebral cortex.
  • To provide a detailed protocol for researchers.

Main Methods:

  • Development of an in-house polysome profiling pipeline.
  • Utilizing sucrose gradient ultracentrifugation for cell fractionation.
  • Analysis of mRNA translational status based on fractionated samples.

Main Results:

  • The study presents a functional polysome profiling pipeline.
  • The pipeline enables assessment of translational efficiency in developing mouse brain cells.
  • Detailed protocols are provided for replication.

Conclusions:

  • Translational regulation is crucial for neural stem cell fate.
  • The developed polysome profiling pipeline is a valuable tool for studying this process.
  • This methodology can advance our understanding of mammalian brain development.