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Updated: Nov 2, 2025

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
Carvacrol-loaded liposome suspension: optimization, characterization and incorporation into poly(vinyl alcohol) films
Adassa Gama Tavares1, Johana Andrade, Rafael Resende Assis Silva
1Department of Food Technology, Federal University of Viçosa, Av. Peter Henry Rolfs, S/N, 36570-900, Viçosa, MG, Brazil. adassa_tavares@hotmail.com.
Abstract:
The purpose of this study was to encapsulate carvacrol into liposomes in order to promote its application in active food packaging. Response surface methodology was used to evaluate the effect of the concentration of the liposomal components on its characteristics. The optimum formulation for the preparation of liposomes with the highest encapsulation efficiency (59.0 ± 1.99%) was found to be 3000 μg mL-1 of cholesterol and 4000 μg mL-1 of carvacrol. Carvacrol reduced the polydispersity index and increased the zeta potential and the thermal stability of liposomes. Fourier-transform infrared spectroscopy indicated that the interaction of carvacrol with liposomes occurred probably through hydrogen-bonding. The incorporation into liposomes maintained the antibacterial effect of carvacrol, but when in the film, carvacrol liposomes were not effective against the microorganisms tested. Liposomes may offer a viable option for stabilizing carvacrol, however, more studies are necessary to enable its application in food packaging.
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