Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.5K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.3K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoelectrochemically homogeneous nickel oxide photocathode composed of nanocrystals prepared by supercritical hydrothermal synthesis.

Nanoscale advances·2026
Same author

Bound states in the continuum in plasmonic structures.

Reports on progress in physics. Physical Society (Great Britain)·2026
Same author

Varifocal Alvarez metalens array for adaptive light-field imaging.

Nature communications·2026
Same author

Coexistence of Metal and Dielectric Resonance Modes in a Single Nanostructure of a Hyperbolic Material.

ACS nano·2026
Same author

Hot Electron-Driven Amide Bond Formation in Plasmonic Nanogaps without Chemical Activation.

The journal of physical chemistry letters·2026
Same author

Design, Synthesis, and Evaluation of Braylin Derivatives as Novel PDE4 Inhibitors with Anti-Inflammatory Effects.

Pharmaceutics·2026

Related Experiment Video

Updated: Nov 2, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.9K

Varifocal Metalens for Optical Sectioning Fluorescence Microscopy.

Yuan Luo1,2,3, Cheng Hung Chu1,4,5, Sunil Vyas1

  • 1Institute of Medical Device and Imaging, National Taiwan University, Taipei 10051, Taiwan.

Nano Letters
|June 7, 2021
PubMed
Summary

Researchers developed a novel Moiré metalens for advanced fluorescence imaging. This tunable lens corrects aberrations, enabling clearer 3D images for biological research and potential clinical applications.

Keywords:
HiLo imagingfluorescence microscopymetasurfaceoptical sectioningtelecentric configuration

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.0K
Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.2K

Related Experiment Videos

Last Updated: Nov 2, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.9K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.0K
Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.2K

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Materials Science

Background:

  • Fluorescence microscopy is crucial for 3D biological imaging.
  • Tunable lenses aid multiplane imaging but often introduce aberrations.
  • Existing methods struggle with image quality in volumetric samples.

Purpose of the Study:

  • To design and implement a dielectric Moiré metalens for aberration-free fluorescence imaging.
  • To develop a varifocal lens system for high-contrast multiplane imaging.
  • To evaluate the metalens performance in biological samples.

Main Methods:

  • Fabrication of a Moiré metalens using complementary phase metasurfaces.
  • Tuning mutual angles to achieve variable focal length (10-125 mm at 532 nm).
  • Integration into a telecentric configuration for HiLo illumination microscopy.

Main Results:

  • Demonstrated aberration correction and distortion reduction in fluorescence images.
  • Achieved high-contrast multiplane imaging of fluorescent beads and ex vivo mouse intestine tissue.
  • Validated the tunable focal length and optical sectioning capabilities.

Conclusions:

  • The Moiré metalens offers a compact and effective solution for advanced fluorescence microscopy.
  • This technology shows promise for improving 3D imaging in biological research.
  • Potential applications include clinical fluorescence microscopy and endoscopy.