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Related Experiment Video

Updated: Nov 2, 2025

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
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A single snapshot multiplex immunoassay platform utilizing dense test lines based on engineered beads.

Wonhyung Lee1, Hojin Kim2, Pan Kee Bae3

  • 1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Cheongam-ro77, Nam-gu, Pohang-si, Gyeongsangbuk-do, 37673, South Korea.

Biosensors & Bioelectronics
|June 7, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic chip for rapid, on-site multiplex diagnosis of infectious diseases. The device simultaneously detects antibodies for COVID-19, Zika, dengue, and chikungunya viruses within 30 minutes.

Keywords:
COVID-19ImmunoassayMicrofluidicsMultiplex assayRapid test

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Area of Science:

  • Biomedical Engineering
  • Infectious Disease Diagnostics
  • Microfluidics

Background:

  • Co-circulation of COVID-19 and dengue necessitates rapid, accurate multiplex diagnostics.
  • Current diagnostic methods may lack the speed and multiplexing capability for emerging pandemic threats.

Purpose of the Study:

  • To develop an innovative microfluidic chip for snapshot multiplex immunoassay.
  • To enable timely on-site detection of multiple viral antibodies.

Main Methods:

  • Engineered microbeads in an open microchannel assembly to create high-density test lines.
  • Developed a pixel intensity-based imaging process for reliable fluorescence signal analysis.
  • Utilized a snapshot multiplex immunoassay on a microfluidic chip.

Main Results:

  • Simultaneous detection of antibodies for arboviruses (Zika, dengue, chikungunya) and SARS-CoV-2 achieved.
  • High-density test lines within a small field-of-view enabled unprecedented multiplexing.
  • Accurate classification of antibody combinations within 30 minutes.

Conclusions:

  • The developed microfluidic chip provides a powerful diagnostic tool for controlling current and future pandemics.
  • Snapshot multiplex immunoassay offers timely on-site response capabilities.
  • The technology enhances diagnostic reliability through advanced imaging processing.