Effect of creating a fed-batch like condition using carbon to nitrogen ratios on lipid accumulation in Rhodosporidium...

Utilizing the undetoxified wood hydrolysate to accumulate maximum lipids in Rhodosporidium toruloides under optimum conditions has been regarded as a renewable and cost-effective strategy. The current investigation aims to identify the best carbon to nitrogen (C/N 20, 70, and 120) ratio for maximum lipid accumulation in R. toruloides-1588 using wood hydrolysate. Additionally, a fed-batch-like condition was employed, where C/N ratios were maintained during the fermentation that inherently decreases in batch fermentation. The C/N ratio 70 has been identified as the best condition with 3 times higher lipid accumulation (43% w/w) than the control. Additionally, >95% and 70% of glucose and xylose consumption were observed, respectively. Moreover, 50% increase in polyunsaturated fatty acids compared to the control media reinforced the potential of R. toruloides-1588 to thrive on undetoxified hydrolysate, high lipid productivity (3.8 mg/g of dry weight per hour) and produce high value monosaturated and polyunsaturated fatty acids.