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Related Experiment Video

Updated: Nov 2, 2025

Highly-Multiplexed Tissue Imaging with Raman Dyes
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Multiplexed live-cell profiling with Raman probes.

Chen Chen1, Zhilun Zhao1, Naixin Qian1

  • 1Department of Chemistry, Columbia University, New York, NY, USA.

Nature Communications
|June 8, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel multiplexed Raman probe panel for detailed single-cell analysis, enabling simultaneous quantification of multiple cellular functions. This advanced technique offers powerful insights for cell biology and drug discovery.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Spectroscopy

Background:

  • Single-cell multiparameter measurement is crucial for understanding complex biological systems.
  • Existing methods face challenges in deciphering numerous phenotypes within a single live cell.

Purpose of the Study:

  • To develop a multiplexed Raman probe panel for simultaneous quantification of multiple cellular parameters in individual live cells.
  • To enable high-content live-cell profiling and phenotyping.

Main Methods:

  • Devised a multiplexed Raman probe panel with sharp, resolvable peaks.
  • Coupled the panel with whole-cell spontaneous Raman micro-spectroscopy.
  • Demonstrated 14-plexed live-cell profiling and phenotyping under drug perturbations.

Main Results:

  • Successfully quantified cell surface proteins, endocytosis activities, and metabolic dynamics simultaneously.
  • Enabled powerful clustering, correlation, and network analysis for biological insights.
  • Showcased utility in live-cell cytometry with low instrument complexity.

Conclusions:

  • The developed Raman probe platform offers a robust and straightforward method for highly multiplexed single-cell measurements.
  • This technique serves as a valuable tool for basic single-cell biology research.
  • It has significant potential for translational applications like high-content cell sorting and drug discovery.