Metabolic control of daily locomotor activity mediated by tachykinin in Drosophila

Metabolism influences locomotor behaviors, but the understanding of neural curcuit control for that is limited. Under standard light-dark cycles, Drosophila exhibits bimodal morning (M) and evening (E) locomotor activities that are controlled by clock neurons. Here, we showed that a high-nutrient diet progressively extended M activity but not E activity. Drosophila tachykinin (DTk) and Tachykinin-like receptor at 86C (TkR86C)-mediated signaling was required for the extension of M activity. DTk neurons were anatomically and functionally connected to the posterior dorsal neuron 1s (DN1_ps) in the clock neuronal network. The activation of DTk neurons reduced intracellular Ca²⁺ levels in DN1_ps suggesting an inhibitory connection. The contacts between DN1_ps and DTk neurons increased gradually over time in flies fed a high-sucrose diet, consistent with the locomotor behavior. DN1_ps have been implicated in integrating environmental sensory inputs (e.g., light and temperature) to control daily locomotor behavior. This study revealed that DN1_ps also coordinated nutrient information through DTk signaling to shape daily locomotor behavior.