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Related Experiment Video

Updated: Nov 2, 2025

Author Spotlight: Robust Culture of Human Enteric Neurons from Human Pluripotent Stem Cells

Author Spotlight: Robust Culture of Human Enteric Neurons from Human Pluripotent Stem Cells

Published on: May 17, 2024

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells.

Antigoni Gogolou¹, Thomas J R Frith², Anestis Tsakiridis¹

¹Centre for Stem Cell Biology, Department of Biomedical Science, The University of Sheffield, Sheffield, United Kingdom.

Current Protocols

|June 8, 2021

Summary

Human pluripotent stem cells (hPSCs) can be differentiated into enteric nervous system (ENS) progenitors and neurons. This protocol offers a promising avenue for studying and treating enteric neuropathies.

Keywords:

ENS progenitors hPSCs human pluripotent stem cells vagal neural crest

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Published on: October 28, 2011

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Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

Area of Science:

Developmental biology
Stem cell biology
Neuroscience

Background:

The enteric nervous system (ENS) regulates gastrointestinal function and homeostasis.
ENS development originates from vagal neural crest (NC) cells.
Defects in ENS development lead to untreatable enteric neuropathies.

Purpose of the Study:

To describe a differentiation strategy for generating enteric neural progenitors and neurons from human pluripotent stem cells (hPSCs).
To establish an in vitro model for studying human ENS development and pathology.
To explore potential cell therapy applications for enteric neuropathies.

Main Methods:

Utilizing a combination of instructive signals and retinoic acid for dose/time-dependent differentiation.
Guiding hPSCs through a vagal neural crest (NC) intermediate.
Culturing cells in neurotrophic media to promote enteric neuron and glia development.

Main Results:

Successfully derived enteric neural progenitors from hPSCs via a vagal NC intermediate.
Demonstrated the differentiation of these progenitors into functional enteric neurons and glia.
Established detailed protocols for hPSC-based ENS cell generation.

Conclusions:

hPSCs provide a viable in vitro source for enteric neurons.
This differentiation strategy facilitates research into ENS development and disease.
The protocol holds potential for future cell-based therapeutic strategies for enteric neuropathies.