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Progressive bovine sperm separation using parallelized microchamber-based microfluidics.

Mohammad Yaghoobi1, Morteza Azizi1, Amir Mokhtare1

  • 1Food Science Department, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA. alireza@cornell.edu.

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|June 9, 2021
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Summary
This summary is machine-generated.

This study introduces a microfluidic device for efficient sperm sorting, significantly improving motility and DNA integrity for infertility treatments. The platform offers a rapid, non-damaging method for selecting high-quality sperm for assisted reproductive technologies.

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Area of Science:

  • Reproductive Biology
  • Biomedical Engineering
  • Microfluidics

Background:

  • Sperm motility is crucial for natural fertilization and assisted reproductive technologies (ARTs).
  • Conventional sperm separation methods like centrifugation can damage sperm cells.
  • Existing microfluidic sperm sorting systems face challenges in efficiency and throughput, particularly for low-concentration samples (oligozoospermia).

Purpose of the Study:

  • To develop and demonstrate a microchamber-based microfluidic platform for efficient separation of progressively motile sperm.
  • To assess the platform's performance in terms of speed, controllability, and sperm quality enhancement.
  • To evaluate the potential of this technology for improving sperm selection in ART.

Main Methods:

  • A microfluidic platform featuring 384 microchambers was utilized for sperm separation.
  • The system sorts progressively motile sperm from non-viable sperm and debris, trapping nonprogressive sperm.
  • No sample preparation was required, and the process was completed in under 10 minutes.

Main Results:

  • The mean average-path velocity of collected motile sperm increased from 57 ± 10 μm/s to 81 ± 13 μm/s.
  • DNA integrity of the separated sperm improved by 20% compared to the raw sample.
  • A bovine sperm sample (22.5 μL, 8.5 M/mL, 38% motility) yielded a concentrated sample (2.1 M/mL, 90% motility) with 75% retrieval efficiency.

Conclusions:

  • The microchamber-based microfluidic platform effectively separates progressively motile sperm with enhanced quality and DNA integrity.
  • The platform demonstrates high throughput potential, as microchamber depth does not limit residence time for motile sperm.
  • This technology offers a promising, robust, and efficient solution for sperm collection in ART, though human studies are needed.