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A rapid computerized method for quantitation of cellular mediated immunity in biological systems
E K Barbour1, J A Newman, J Sasipreeyajan
1Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Abstract:
The aim of this study was to compare the quantitation of cellular mediated immunity (CMI) assessed by leukocyte migration (LM) using a Hewlett Packard Model 9825A Desk-top computer interfaced to a Talos Cyber Graphics Digitizer vs. the standard quantitation done by tracing the projected profile of the migrated leukocytes on papers, cutting and weighing. The computerized quantitation resulted in a percentage migration mean of 75.91 as compared to standard quantitation by weighing (76.37) (P greater than 0.05). The unweighted least squares linear regression resulted in R2 0.9987, an indication of a high correlation between the data obtained by both methods. The advantages of computerized quantitation of cellular mediated immunity are discussed.
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