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TURBOLYTIK: a peptide cleavage program for personal computers.

T S Pillay1

  • 1Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.

International Journal of Bio-Medical Computing
|May 1, 1988
PubMed
Summary

This study introduces a new microcomputer program that simulates polypeptide cleavage, aiding protein chemistry research. The software predicts peptide fragments, molecular weights, and isoelectric points for easier analysis.

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Area of Science:

  • Biochemistry
  • Computational Biology
  • Bioinformatics

Background:

  • Protein structure analysis is crucial in biochemistry.
  • Identifying peptide fragments aids in understanding protein function and modifications.
  • Current methods for predicting cleavage sites can be complex.

Purpose of the Study:

  • To describe a novel microcomputer program for simulating polypeptide cleavage.
  • To provide a tool for predicting cleavage fragments, molecular weights, and isoelectric points.
  • To assist in selecting proteolytic methods and identifying peptides.

Main Methods:

  • The program utilizes Turbo Basic for MS-DOS personal computers.
  • It simulates chemical and enzymic cleavage of polypeptides based on primary structure.

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  • Calculates molecular weights, charge at a given pH, and predicts isoelectric points.

    • Main Results:

    • The program generates all possible cleavage fragments for a given protein.
    • It provides estimations of molecular weights and charge per molecule.
    • Isoelectric points are predicted for the generated peptide fragments.

    Conclusions:

    • This simulation program simplifies the selection of proteolytic methods in protein chemistry.
    • It aids in identifying peptides on peptide maps and in mass spectrometry data.
    • The software is valuable for defining posttranslational modifications and verifying primary structures from cDNA.