Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.2K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic value of [<sup>18</sup>F]fluorodeoxyglucose PET/CT in esophageal cancer patients treated with anti-PD-1 inhibitors.

BMC medical imaging·2026
Same author

Prepatterned Superconducting Contacts for Clean Superconductor-Topological Material Interfaces Enabling Long-Range Josephson Coupling.

Nano letters·2026
Same author

Efficacy, Safety, and Economic Evaluation of Gamidaeganghwal-Tang in Patients with Knee Osteoarthritis: Protocol for a Multicenter, Randomized, Double-Blind, Controlled Trial.

Journal of pain research·2026
Same author

Vacancy-Induced Z-Contrast Anomaly in Self-Assembled (Ti,V)O<sub>2</sub> Heterostructure.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Correlation Between Clinical Characteristics and Image Parameters of Preoperative <sup>18</sup>F-fluorocholine PET/CT in Hyperparathyroidism Patients.

Nuclear medicine and molecular imaging·2026
Same author

Sustainable, solvent-free exfoliation of 2D materials for thermally conductive metal powder coatings.

NPJ 2D materials and applications·2026

Related Experiment Video

Updated: Nov 2, 2025

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators
11:44

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

Published on: August 15, 2014

10.5K

TEM sample preparation using micro-manipulator for in-situ MEMS experiment.

Hyunjong Lee1, Odongo Francis Ngome Okello2, Gi-Yeop Kim2

  • 1Korea Institute of Industrial Technology, Incheon, 21999, South Korea.

Applied Microscopy
|June 9, 2021
PubMed
Summary

We developed a new optical microscopy system for easier and safer transmission electron microscopy (TEM) sample preparation. This method efficiently transfers various nano-scale samples onto micro-electro mechanical systems (MEMS) chips.

Keywords:
In-situ transmission electron microscopyMEMSManipulator

More Related Videos

A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens
07:15

A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens

Published on: June 2, 2017

9.4K
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
10:39

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Published on: February 19, 2018

10.9K

Related Experiment Videos

Last Updated: Nov 2, 2025

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators
11:44

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

Published on: August 15, 2014

10.5K
A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens
07:15

A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens

Published on: June 2, 2017

9.4K
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
10:39

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Published on: February 19, 2018

10.9K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Microscopy

Background:

  • In-situ transmission electron microscopy (TEM) is crucial for observing nano-scale dynamics.
  • Current TEM sample preparation methods for in-situ studies are often complex and unsafe.
  • There is a need for improved techniques for preparing samples for dynamic TEM analysis.

Purpose of the Study:

  • To develop a simple and safe method for preparing transmission electron microscopy (TEM) samples for in-situ observation.
  • To introduce an optical microscopy-based micro-manipulating system for efficient sample transfer.
  • To demonstrate the transfer of diverse nano-scale samples onto micro-electro mechanical systems (MEMS) chips.

Main Methods:

  • Development of an optical microscopy-based micro-manipulating system.
  • Utilizing the system for transferring various nano-scale samples, including nanowires and thin plates.
  • Investigating the role of electrostatic force control during the sample transfer process.

Main Results:

  • Successfully transferred multiple types of nano-scale samples (nanowires, plate-like thin samples) onto MEMS chips in a single step.
  • The micro-manipulating system proved effective for in-situ TEM sample preparation.
  • Control of electrostatic force was identified as a critical factor for successful sample transfer.

Conclusions:

  • The developed optical microscopy-based micro-manipulating system offers a simple and safe solution for in-situ TEM sample preparation.
  • This technique facilitates the study of nano-scale phenomena dynamics by enabling efficient sample transfer.
  • Precise control over electrostatic forces is essential for optimizing the sample transfer process in TEM analysis.