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Updated: Jul 24, 2026

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
Delivering embryos following 10 years of cryopreservation, using unpaired freeze/thaw techniques: A case report
Priscila Melantonio1, Jessica Attivo1, Alecsandra P Gomes1
1Monteleone Centro de Reprodução Humana, São Paulo, SP, Brazil.
Abstract:
Although frozen embryo transfer is a widely established route for assisted reproduction, successful frozen embryo transfer using embryos that have undergone long term cryopreservation remains relatively unexplored, and its efficacy remains a matter of some debate. This case report describes two successful frozen embryo transfer conceptions in the same patient, one after 3 months of cryopreservation and the second 10 years after cryopreservation. These embryos were cryopreserved using the slow freezing technique and were thawed using an unpaired technique (ultra-rapid warming) after 10 years of storage.

