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Isolation of antifungal-resistant yeasts from bulk milk in Japan

Ryoka Miyake1, Takakaki Ito2, Hiroshi Kamata3

  • 1Veterinary Dermatology, Nihon University College of Bioresource Sciences, 1866 Kameino, Fusisawa, Kanagawa 252-0880, Japan.

The Journal of Veterinary Medical Science
|June 10, 2021
PubMed

Insights

We found human pathogenic yeasts, including Candida species, in Japanese bulk milk. Some isolates showed resistance to common antifungal drugs like fluconazole and amphotericin B.

Area of Science:

  • Microbiology
  • Mycology
  • Infectious Diseases

Background:

  • The genus Candida comprises numerous species, some of which are opportunistic human pathogens.
  • Bulk milk can serve as a potential reservoir for various microorganisms, including yeasts.
  • Antifungal drug resistance in Candida species is a growing global health concern.

Purpose of the Study:

  • To isolate and identify human pathogenic yeasts, particularly Candida species, from bulk milk samples in Japan.
  • To determine the antifungal drug susceptibility profiles of the isolated yeast strains.
  • To investigate the prevalence of antifungal resistance in these environmental isolates.

Main Methods:

  • Isolation of yeasts from bulk milk samples collected in the Aichi area, Japan.
  • Identification of yeast isolates to the species level.
  • Determination of minimum inhibitory concentrations (MICs) using E-tests for various antifungal drugs.

Main Results:

  • Seven human pathogenic yeast species (14 isolates) were identified, including five Candida species and two related species.
  • Two isolates of Candida albicans and Candida inconspicua exhibited resistance to fluconazole.
  • One Candida krusei isolate was resistant to both fluconazole and itraconazole, and one Candida catenulata isolate showed potential resistance to amphotericin B.

Conclusions:

  • Bulk milk in Japan harbors human pathogenic yeasts, including species of clinical significance.
  • The presence of antifungal-resistant Candida isolates in bulk milk highlights a potential public health risk.
  • Further research is needed to understand the transmission dynamics and implications of antifungal resistance in environmental yeast populations.
Keywords:
Candidaantifungal resistancebulk milkcowsusceptibility testing

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