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Mass spore production of Mucor circinelloides on rice

J Alberto Patiño-Medina1, Viridiana Alejandre-Castañeda1, Marco I Valle-Maldonado1,2

  • 1Laboratorio de Diferenciación Celular, Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria 58030, Morelia, Michoacán México.

3 Biotech
|June 10, 2021
PubMed

Insights

Rice grains offer an inexpensive method for large-scale Mucor circinelloides sporangiospore production. Spores grown on rice show similar characteristics and virulence to those grown on traditional media, making rice a viable alternative substrate.

Area of Science:

  • Mycology
  • Biotechnology
  • Fungal spore production

Background:

  • Mucor circinelloides produces diverse spores, with sporangiospores being key asexual reproductive units.
  • The fungus holds potential for various biotechnological applications.

Purpose of the Study:

  • To evaluate rice (Oryza sativa) grains as a cost-effective substrate for mass production of M. circinelloides sporangiospores.

Main Methods:

  • Culturing M. circinelloides on rice grains and comparing sporangiospore characteristics with those from yeast extract-peptone-glucose (YPG) medium.
  • Assessing protein and nucleic acid content, germination phenotypes, and virulence against Caenorhabditis elegans.
  • Evaluating plasmid stability in transgenic M. circinelloides strains sporulated on rice.

Main Results:

  • Sporangiospores from rice and YPG medium showed comparable protein and nucleic acid content.
  • Similar germination patterns and virulence rates against C. elegans were observed for spores from both substrates.
  • Transgenic strains on rice maintained plasmid stability, with a minor loss (approx. 20%) after the first passage.

Conclusions:

  • Rice is a suitable and inexpensive substrate for the large-scale production of M. circinelloides sporangiospores.
  • The use of rice does not significantly alter key spore characteristics or virulence.
  • Rice offers a practical alternative for fungal cultivation in biotechnological contexts.
Keywords:
MucoralSpore productionSpore propagationSporulation

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