Related Experiment Video

Updated: Nov 2, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.8K

MicroRNA-340-5p inhibits the malignant phenotypes of osteosarcoma by directly targeting NRF2 and deactivating the

H-P Fan1, S-Y Wang, Y-Y Shi

  • 1Department of Pediatrics, Yantai Maternity and Child Care Hospital, Yantaishan Hospital, Yantai, China. xiangkangbuxing540@163.com.

European Review for Medical and Pharmacological Sciences
|June 10, 2021
PubMed
Abstract

Insights

MicroRNA-340-5p (miR-340-5p) is downregulated in Osteosarcoma (OS) and inhibits tumor progression by targeting NRF2. Restoring miR-340-5p shows therapeutic potential for OS.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with aggressive progression.
  • MicroRNAs (miRNAs) play critical roles in cancer development and progression.
  • The specific role of miR-340-5p in OS pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the effects of miR-340-5p on Osteosarcoma progression.
  • To explore the underlying molecular mechanisms of miR-340-5p in OS.
  • To assess the therapeutic potential of miR-340-5p in OS treatment.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for gene expression analysis.
  • Cell viability, invasion, and migration assays (MTT, Transwell) to assess OS cell behavior.
  • Bioinformatic prediction (TargetScan) and luciferase reporter assays to identify miR-340-5p targets.
  • Western blot analysis to examine protein expression related to PI3K/AKT pathway and epithelial-mesenchymal transition (EMT).

Main Results:

  • miR-340-5p expression was significantly decreased in OS tissues and cells.
  • Lower miR-340-5p levels correlated with poor prognosis and advanced clinicopathologic features in OS patients.
  • Upregulation of miR-340-5p suppressed OS cell proliferation, invasion, and migration.
  • miR-340-5p directly targets NRF2, inhibiting the PI3K/AKT pathway and EMT.

Conclusions:

  • miR-340-5p acts as a tumor suppressor in Osteosarcoma by targeting NRF2.
  • miR-340-5p represses malignant phenotypes through regulation of the PI3K/AKT pathway and EMT.
  • The miR-340-5p/NRF2 axis presents a promising therapeutic target for Osteosarcoma.

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.6K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.8K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K