Complete Genome Sequence of Escherichia coli Antibiotic Resistance Isolate Bank Number 0346

Area of Science:

• Microbiology
• Genomics
• Public Health

Background:

• Plasmid-mediated polymyxin resistance, particularly the mcr-1 gene, poses a significant public health threat.
• The rapid horizontal transfer of resistance genes contributes to the spread of antibiotic resistance.

Purpose of the Study:

• To characterize the complete genome of a colistin-resistant Escherichia coli isolate harboring the mcr-1 gene.
• To provide genomic data for understanding the dissemination of polymyxin resistance.

Main Methods:

• Whole-genome sequencing of the Escherichia coli isolate (Antibiotic Resistance Isolate Bank number 0346).
• Bioinformatic analysis to identify the mcr-1 gene and its plasmid context.

Main Results:

• The complete genome sequence of the colistin-resistant Escherichia coli isolate was determined.
• The presence of the plasmid-borne mcr-1 gene was confirmed in the isolate.

Conclusions:

• The genomic data provides a basis for studying the epidemiology of mcr-1.
• Understanding the genetic context of mcr-1 is crucial for combating polymyxin resistance in clinical settings.