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Published on: May 19, 2023
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PFKFB3-dependent glucose metabolism regulates 3T3-L1 adipocyte development
Beth A Griesel1, Satoshi Matsuzaki2, Albert Batushansky2
1Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Summary
Early glucose availability is crucial for adipocyte differentiation. Supplementing differentiation media with 5 mM glucose enhances glucose uptake, expands the metabolome, and upregulates key proteins like GLUT4 and adiponectin.
Area of Science:
- Cell Biology
- Metabolism
- Biochemistry
Background:
- Adipocyte differentiation involves increased glucose uptake.
- High glucose is required only during the initial 3 days of in vitro differentiation.
- Specific glucose metabolic pathways driving adipocyte differentiation remain unclear.
Purpose of the Study:
- To investigate glucose metabolism during the initial 3 days of 3T3-L1 adipocyte differentiation.
- To identify the specific glucose metabolic pathways essential for adipocyte maturation.
- To determine the role of glucose concentration in adipocyte differentiation and proteome expansion.
Main Methods:
- Utilized 3T3-L1 adipocytes as a model system.
- Compared differentiation with and without 5 mM glucose in the culture media.
- Employed genetic and pharmacologic approaches to assess the role of PFKFB3.
Main Results:
- Differentiation cocktail alone activated glucose uptake and glycolysis but not GLUT4 or adiponectin expression.
- 5 mM glucose promoted transient glucose uptake and glycolysis, expanding the adipocyte metabolome and proteome.
- Increased 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) expression mediated the positive effects of glucose.
Conclusions:
- Glucose availability during the first 3 days of differentiation is critical for adipocyte maturation.
- PFKFB3 is a key regulator of glycolysis and metabolic remodeling essential for adipocyte differentiation.
- Targeting PFKFB3 may influence adipocyte function and metabolic health.
Keywords:
adipogenesisadiponectinglucose metabolismglucose transportglucose transporter 4 (GLUT4)phosphofructokinase fructose bisphosphatase 3 (PFKFB3)More Related Videos
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