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Cell Washing and Solution Exchange in Droplet Microfluidic Systems.

Can Huang1, Han Zhang1, Song-I Han1

  • 1Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas 77843, United States.

Analytical Chemistry
|June 10, 2021
PubMed
Summary

This study introduces a novel droplet microfluidic technology for efficient cell washing and solution exchange. The method uses dielectrophoretic (DEP) force to achieve high solution exchange rates with minimal cell loss, advancing cell-based assays.

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Area of Science:

  • Microfluidics
  • Cell Biology
  • Biotechnology

Background:

  • Droplet microfluidics enable most cell handling steps.
  • Cell washing and solution exchange remain a challenge in droplet microfluidics.

Purpose of the Study:

  • To develop an in-droplet cell washing and solution exchange technology.
  • To integrate sequential droplet manipulation steps into a single platform.

Main Methods:

  • Utilizing dielectrophoretic (DEP) force for cell manipulation.
  • Asymmetrical droplet splitting and merging with new solutions.
  • Demonstrated on microalgae and bacteria cell manipulation.

Main Results:

  • Achieved up to 88% solution exchange with less than 5% cell loss.

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  • Successfully induced lipid production in microalgae by medium exchange.
  • Improved detection sensitivity in bacteria assays by removing fluorescent noise.

    • Conclusions:

    • The developed technology enables efficient cell washing and solution exchange in droplet microfluidics.
    • This advancement broadens the scope of assays performable in droplet microfluidic formats.
    • Potential applications in cell biology, diagnostics, and synthetic biology.