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Fluid Dynamics of the Open Port Interface for High-Speed Nanoliter Volume Sampling Mass Spectrometry
Chang Liu1, Gary J Van Berkel2, Peter Kovarik1
1SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4V8, Canada.
Analytical Chemistry
|June 10, 2021
Summary
The open port interface (OPI) enables rapid, high-volume sample transfer for mass spectrometry. This technology achieves faster analysis by minimizing carry-over and matrix effects, enhancing analytical throughput.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Fluid Dynamics
Background:
- High-throughput mass spectrometry requires efficient sample introduction.
- Ionization suppression from sample matrices limits analytical sensitivity and speed.
- Existing methods struggle with rapid, diluted sample transfer.
Purpose of the Study:
- To optimize the open port interface (OPI) for high-speed nanoliter sample droplet transfer.
- To investigate the fluid dynamics governing sample transport and dilution.
- To enhance mass spectrometric analysis through improved sample handling.
Main Methods:
- Utilized computational fluid dynamics (CFD) simulations and analytic calculations.
- Correlated simulation data with experimental measurements and photographic analysis.
- Modified geometric, flow, and dispensing parameters of the OPI system.
Main Results:
- Demonstrated sample transfer rates of at least 15 Hz, exceeding previous 1 Hz optimization.
- Achieved sample dilution greater than 1000-fold to mitigate matrix effects.
- Established conditions for contact-free sample transfer, significantly reducing carry-over.
Conclusions:
- The OPI system, particularly with acoustic ejection mass spectrometry (AEMS), offers a unique approach to flow injection analysis.
- Optimized OPI design enables rapid, diluted, and low-carryover sample introduction for mass spectrometry.
- This advancement facilitates higher analytical throughput and improved data quality in mass spectrometry applications.
