The influence of the support material used on band sharpness in Immobiline gels

To achieve excellent isoelectrofocusing results with the use of Immobiline gels, the quality of the support is essential. Effects such as diffuse bands, missing protein fractions, streaking and trailing could originate from a support of inadequate quality. Two different treatments to eliminate these effects were evaluated. A washing procedure improves the band sharpness, and addition of carrier ampholytes eliminates streaking and trailing.