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Updated: Nov 2, 2025
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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
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IMAGING ARTIFACTS IN FLUORESCENCE LIFETIME IMAGING OPHTHALMOSCOPY.
Chantal Dysli1,2, Muriel Dysli1,2, Joel Lincke1,2
1Department of Ophthalmology, Inselspital, Bern University Hospital, Bern, Switzerland; and.
Retina (Philadelphia, Pa.)
|June 10, 2021
Summary
Imaging artifacts significantly impact retinal fluorescence lifetime (FLIO) measurements. Proper technique is crucial for accurate FLIO analysis, avoiding issues like short acquisition times or ocular surface problems.
Area of Science:
- Ophthalmology
- Biomedical Optics
- Medical Imaging
Background:
- Retinal fluorescence lifetime imaging (FLIO) offers a novel method for assessing ocular health.
- Understanding factors affecting FLIO image quality is essential for reliable clinical application.
Purpose of the Study:
- To quantify the impact of imaging artifacts on retinal fluorescence lifetime (FLIO) values.
- To provide guidance on artifact avoidance and FLIO image acquisition quality improvement.
Main Methods:
- A systematic experimental imaging study was conducted on five healthy human eyes.
- A fluorescence lifetime imaging ophthalmoscope was utilized for data acquisition.
- Quantitative analysis assessed FLIO lifetime changes caused by specific artifacts.
Main Results:
- Artifacts such as insufficient acquisition time, poor illumination, ocular surface issues, and defocus significantly altered FLIO values.
- Prior fluorescein administration (systemic or topical) precludes retinal FLIO analysis.
Conclusions:
- Awareness of potential artifact sources is critical for accurate FLIO acquisition and interpretation.
- Standardized procedures for FLIO imaging and analysis are necessary for high-quality, quantitative assessments.
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