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CircPVT1 promotes proliferation of lung squamous cell carcinoma by binding to miR-30d/e

Jie Shi1, Xin Lv1, Lizhong Zeng1

  • 1Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, 157th Xiwu Road, Xi'an, 710000, People's Republic of China.

Journal of Experimental & Clinical Cancer Research : CR
|June 11, 2021
PubMed
Abstract

Insights

Circular RNA PVT1 (circPVT1) promotes lung squamous cell carcinoma (LUSC) progression by interacting with HuR and acting as a ceRNA for miR-30d/e to regulate CCNF. CircPVT1 may serve as a diagnostic biomarker for LUSC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are emerging regulators in human diseases, including cancer.
  • The role and mechanisms of circRNAs in lung squamous cell carcinoma (LUSC) are not well understood.

Purpose of the Study:

  • To investigate the expression, function, and clinical significance of circPVT1 in LUSC.
  • To elucidate the molecular mechanisms underlying circPVT1's role in LUSC progression.

Main Methods:

  • RNA sequencing (RNA-seq) and qRT-PCR for expression profiling.
  • In vitro and in vivo experiments to assess circPVT1's functional impact.
  • Molecular assays including RNA pull-down, mass spectrometry, and dual-luciferase reporter assays to determine interactions.

Main Results:

  • circPVT1 was significantly upregulated in LUSC tissues, serum, and cell lines.
  • Higher circPVT1 expression correlated with poorer survival rates in LUSC patients.
  • circPVT1 promotes LUSC cell proliferation and tumor growth by regulating the HuR/circPVT1/miR-30d/e/CCNF axis.

Conclusions:

  • circPVT1 promotes LUSC progression through a complex regulatory cascade involving HuR and the ceRNA network of miR-30d/e and CCNF.
  • circPVT1 represents a potential diagnostic biomarker and therapeutic target for LUSC.
Keywords:
CCNFHuRLung squamous cell carcinomacircPVT1miR-30

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