Related Experiment Video
Updated: Nov 2, 2025
09:31
Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS
Published on: August 31, 2017
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Reductive dissolution of pyrite by methanogenic archaea.
Devon Payne1, Rachel L Spietz1, Eric S Boyd2
1Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA.
The ISME Journal
|June 11, 2021
Summary
Methanogens can break down pyrite (FeS2) at low temperatures, accessing essential iron and sulfur. This finding reveals a more complex microbial role in ancient and modern anoxic environments.
Area of Science:
- Biogeochemistry
- Microbiology
- Geochemistry
Background:
- Pyrite (FeS2) formation and fate significantly impact global biogeochemical cycles.
- Traditionally, FeS2 was considered stable and unavailable to microbes in anoxic, low-temperature environments.
Purpose of the Study:
- To investigate the bioavailability and microbial utilization of pyrite (FeS2) by methanogens under anoxic, low-temperature conditions.
- To reassess the role of microbial activity in iron and sulfur cycling.
Main Methods:
- Experimental incubation of methanogens with pyrite (FeS2) at temperatures up to 38°C.
- Analysis of cellular iron and sulfur assimilation and FeS2 reductive dissolution.
Main Results:
- Methanogens catalyze the reductive dissolution of FeS2 at low temperatures (≤38°C).
- Microbial cells directly access FeS2 for iron and sulfur assimilation, supporting cofactor biosynthesis.
- Iron monosulfide formation observed, suggesting coupled reductive dissolution and precipitation.
Conclusions:
- Pyrite (FeS2) is bioavailable to anaerobic methanogens, challenging previous paradigms.
- Methanogens mobilize FeS2 in low-temperature anoxic environments, impacting iron and sulfur cycles.
- Microbial contributions to ancient and modern biogeochemical cycles are more complex than previously understood.
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