Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

5.1K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.1K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

4.7K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.7K
The Cell Cycle Control System01:28

The Cell Cycle Control System

4.4K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
4.4K
The Cell Cycle Control System02:11

The Cell Cycle Control System

13.0K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
13.0K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.8K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.8K
Positive Regulator Molecules02:39

Positive Regulator Molecules

6.0K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Hemodynamic Performance of Two Mechanical Cardiopulmonary Resuscitation Devices.

ASAIO journal (American Society for Artificial Internal Organs : 1992)·2026
Same author

Pre-hospital neurological risk stratification at return of spontaneous circulation (ROSC) after out-of-hospital cardiac arrest - the Pre-MIRACLE<sub>2</sub> score.

Resuscitation·2026
Same author

The FaciLItated hospital-based ECPR via Helicopter Transport (FLIGHT-to-ECPR) study.

Resuscitation·2026
Same author

Optimal Fluoroscopic Angles for Percutaneous Coronary Intervention During Mechanical Cardiopulmonary Resuscitation in Cardiac Arrest Patients.

Interventional cardiology (London, England)·2026
Same author

Endovascular resuscitation: an expert practice review.

Emergency medicine journal : EMJ·2026
Same author

The Minnesota Mobile Resuscitation Consortium ECMO truck: a feasibility study for ECPR in refractory out-of-hospital cardiac arrest.

Resuscitation·2026

Related Experiment Video

Updated: Nov 2, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

27.8K

CDK control pathways integrate cell size and ploidy information to control cell division.

James Oliver Patterson1,2, Souradeep Basu1, Paul Rees2,3

  • 1Cell Cycle Laboratory, The Francis Crick Institute, London, United Kingdom.

Elife
|June 11, 2021
PubMed
Summary

Cell size control relies on cyclin-dependent kinase (CDK) activity. In fission yeast, CDK inhibitory phosphorylation and PP2A phosphatase ensure proper cell division timing, preventing smaller cells from dividing.

Keywords:
CDKPP2AS. pombecell biologycell cyclecell size controlcomputational biologycyclinsynthetic biologysystems biology

More Related Videos

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:05

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

209
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.5K

Related Experiment Videos

Last Updated: Nov 2, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

27.8K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:05

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

209
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.5K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell size homeostasis is crucial for eukaryotic cell function.
  • Cell division must be coordinated with cell growth to maintain size.
  • Cyclin-dependent kinase (CDK) activity is central to regulating mitosis and cell size.

Purpose of the Study:

  • To investigate how cell size information is processed by the cell cycle network in fission yeast.
  • To dissect the in vivo determinants of CDK activity related to cell size.

Main Methods:

  • Development of a high-throughput single-cell assay for CDK activity in vivo.
  • Analysis of CDK inhibitory phosphorylation and the role of phosphatase PP2A.
  • Comparison of CDK activity in haploid and diploid cells.

Main Results:

  • Inhibitory tyrosine phosphorylation of CDK encodes cell size information.
  • The phosphatase PP2A contributes to setting a division size threshold.
  • Diploid cells show reduced CDK activity compared to haploid cells of equivalent size, indicating DNA-dependent inhibition.

Conclusions:

  • CDK inhibitory phosphorylation and PP2A act synergistically to prevent premature mitosis in smaller cells.
  • DNA levels can inhibit CDK activity, further contributing to cell size homeostasis.
  • Multiple mechanisms, including cyclin-CDK scaling, phosphorylation, PP2A, and DNA-dependent inhibition, regulate cell size in fission yeast.