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Bead-Based Assays for Validating Proteomic Profiles in Body Fluids.

Annika Bendes1, Matilda Dale1, Cecilia Mattsson1

  • 1Science for Life Laboratory, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2021
PubMed
Summary
This summary is machine-generated.

Developing robust protein biomarker assays is crucial for clinical applications. This study introduces a versatile pipeline for parallel development of multiple sandwich immunoassays using bead-based technology, streamlining biomarker validation.

Keywords:
AntibodyMass spectrometry (MS)Protein standardSandwich immunoassay (SIA)Single-binder immunoassaysSuspension bead array (SBA)

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Area of Science:

  • Biochemistry
  • Proteomics
  • Clinical Diagnostics

Background:

  • Protein biomarkers in biological fluids are vital for disease management.
  • High-throughput proteomics identifies numerous candidate biomarkers, necessitating validation.
  • Assay performance is critical for reliable biomarker quantification and clinical translation.

Purpose of the Study:

  • To present a versatile pipeline for systematic and parallel development of sandwich immunoassays.
  • To address the challenges of labor and resource intensity in validating multiple protein biomarker candidates.
  • To facilitate the transition of biomarker discoveries from research to routine clinical analysis.

Main Methods:

  • Utilized a bead-based technology for assay development.
  • Implemented a pipeline for systematic and parallel development of sandwich immunoassays.
  • Focused on enabling efficient validation of multiple candidate protein biomarkers.

Main Results:

  • Demonstrated a versatile pipeline for developing multiple sandwich immunoassays simultaneously.
  • Facilitated the systematic and parallel development process for protein quantification assays.
  • Provided a method to overcome challenges in validating numerous biomarker candidates.

Conclusions:

  • The described pipeline offers a streamlined approach to developing and validating multiple protein biomarker assays.
  • This bead-based technology enhances the efficiency and reproducibility of assay development for clinical proteomics.
  • The versatile pipeline supports the advancement of protein biomarker discovery towards routine clinical use.