Related Experiment Video
Updated: Nov 2, 2025
Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
Published on: September 20, 2024
Affinity Proteomics Assays for Cardiovascular and Atherosclerotic Disease Biomarkers
Maria Jesus Iglesias1,2, Jochen M Schwenk3, Jacob Odeberg3,4,5
1Science for Life Laboratory, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Solna, Sweden. mariajesus.iglesias@scilifelab.se.
Insights
This study introduces a novel method for discovering protein biomarkers in human plasma, crucial for early cardiovascular disease diagnosis and risk prediction. The technique is efficient, requires minimal sample, and aids in developing clinical assays.
Area of Science:
- Biochemistry
- Proteomics
- Biomarker Discovery
Background:
- Cardiovascular disease (CVD) involves complex pathophysiological processes impacting the plasma proteome.
- The human plasma proteome offers a rich source for identifying novel disease biomarkers.
- Early diagnosis and risk prediction are critical for managing CVD.
Purpose of the Study:
- To describe a protocol for discovering protein biomarker candidates in human plasma.
- To present a scalable and efficient method for proteomic analysis.
- To facilitate the translation of identified biomarkers into clinical applications.
Main Methods:
- Utilizing suspension bead array technology for high-throughput proteomic analysis.
- Employing a protocol that bypasses the need for abundant protein depletion.
- Using minimal sample volumes (microliters) for plasma analysis.
- Describing a bead-assisted antibody-labeling process for quantitative assay development.
Main Results:
- A protocol for protein biomarker discovery in human plasma was successfully detailed.
- The method demonstrates scalability for analyzing numerous analytes and samples.
- The approach allows for quantitative assay development for biomarker validation.
Conclusions:
- The described protocol offers a powerful tool for identifying novel protein biomarkers for cardiovascular disease.
- This method supports efficient and cost-effective biomarker discovery and validation.
- The technology facilitates the translation of research findings into clinical practice for improved CVD management.
Abstract:
Systematic exploration of the dynamic human plasma proteome enables the discovery of novel protein biomarkers. Using state-of-the-art technologies holds the promise to facilitate a better diagnosis and risk prediction of diseases. Cardiovascular disease (CVD) pathophysiology is characterized for unbalancing of processes such as vascular inflammation, endothelial dysfunction, or lipid profiles among others. Such processes have a direct impact on the dynamic and complex composition of blood and hence the plasma proteome. Therefore, the study of the plasma proteome comprises an excellent exploratory source of biomarker research particularly for CVD. We describe the protocol for performing the discovery of protein biomarker candidates using the suspension bead array technology. The process does not require depletion steps to remove abundant proteins and consumes only a few microliters of sample from the body fluid of interest. The approach is scalable to measure many analytes as well as large numbers of samples. Moreover, we describe a bead-assisted antibody-labeling process that helps to develop quantitative assays for validation purposes and facilitate the translation of the identified candidates into clinical studies.
More Related Videos
07:29Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
08:01Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis
Published on: November 17, 2017
Related Concept Videos
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
01:27Atherosclerosis II: Clinical Manifestations and Diagnostic Tests
01:25Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...