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Updated: Nov 2, 2025
Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
Published on: January 19, 2024
Live-Cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine
Sander Grefte1, Werner J H Koopman2,3
1Human and Animal Physiology, Wageningen University & Research, Wageningen, Netherlands.
Abstract:
Reactive oxygen species (ROS) play an important role in cellular (patho)physiology. Empirical evidence suggests that mitochondria are an important source of ROS, especially under pathological conditions. Here, we describe a method for ROS measurement using dihydroethidium (HEt) and live-cell microscopy.
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