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Updated: Nov 2, 2025
Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
Published on: November 5, 2013
Time-Resolved Imaging of Mitochondrial Flavin Fluorescence and Its Applications for Evaluating the Oxidative State in
A Marcek Chorvatova1,2, M Cagalinec3,4,5, D Chorvat6
1Department of Biophotonics, International Laser Centre, Slovak Centre of Scientific and Technical Information, Bratislava, Slovakia. Alzbeta.MarcekChorvatova@ilc.sk.
Abstract:
Time-resolved fluorescence spectrometry is a highly valuable technological tool to detect and characterize mitochondrial metabolic oxidative changes by means of endogenous fluorescence. Here, we describe detection and measurement of endogenous mitochondrial flavin fluorescence directly in living cardiac cells using fluorescence lifetime imaging microscopy (FLIM) after excitation with 473 nm picoseconds (ps) laser. Time-correlated single photon counting (TCSPC) method is employed.
