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Related Experiment Video

Updated: Nov 2, 2025

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

30.2K

Cellular cholesterol and how to find it.

Valentin Schoop1, Andrea Martello2, Emily R Eden2

  • 1Heidelberg University Biochemistry Center (BZH), 69120 Heidelberg, Germany.

Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids
|June 12, 2021
PubMed
Summary

This review explores methods for tracking cholesterol's location and movement within cells. Understanding these cellular cholesterol transport pathways is vital for treating related diseases.

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Key challenges and recommendations for defining organelle membrane contact sites.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Cholesterol is crucial for eukaryotic cell membrane structure and function.
  • Understanding cholesterol's subcellular localization and transport is key to addressing cholesterol-related diseases.

Purpose of the Study:

  • To provide an overview of methods used to study subcellular cholesterol localization and transport.
  • To guide researchers in selecting appropriate methods for their cholesterol transport studies.

Main Methods:

  • Analysis of cholesterol metabolism via downstream effects (e.g., esterification).
  • Utilizing cholesterol-binding molecules as probes for sterol visualization and quantification.
  • Employing cholesterol analogues to study endogenous sterol transport dynamics.
Keywords:
Cholesterol analoguesFluorescence microscopyIntracellular traffickingLipid transportOrganelle contact sites

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Related Experiment Videos

Last Updated: Nov 2, 2025

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

30.2K
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

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High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
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High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

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Main Results:

  • Methods discussed offer insights into cholesterol metabolism and localization.
  • Specific probes and analogues aid in visualizing and quantifying cellular sterols.
  • Each method presents unique challenges and advantages for studying cholesterol transport.

Conclusions:

  • A comprehensive understanding of available methods is essential for effective research in cholesterol transport.
  • This review aids researchers in choosing the most pertinent techniques for their specific problems.
  • Advancements in methodology are critical for progress in understanding and treating cholesterol-related disorders.