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Published on: June 28, 2024
Quantitative detection of aflatoxin B1 using quantum dots-based immunoassay in a recyclable gravity-driven
Xinran Xiang1, Qinghua Ye2, Yuting Shang2
1Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
A novel polydimethylsiloxane microfluidic chip enables rapid and sensitive detection of aflatoxin B1 (AFB1). This reusable chip offers high specificity and a low detection limit for accurate AFB1 quantification.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Aflatoxin B1 (AFB1) poses significant health risks.
- Sensitive and rapid detection methods for AFB1 are crucial for food safety.
Purpose of the Study:
- To develop a rapid and sensitive detection method for AFB1.
- To create a reusable microfluidic chip for AFB1 analysis.
Main Methods:
- Development of a polydimethylsiloxane gravity-driven cyclic microfluidic chip.
- Utilized a two-signal mode strategy with two-wavelength quantum dot ratio fluorescence.
- Theoretical analysis of the chip's 3D reaction interface.
Main Results:
- Achieved a wide analytic range (0.2-500 ng mL⁻¹).
- Demonstrated a low detection limit (0.06 ng mL⁻¹).
- Exhibited high specificity, good precision (CV < 5%), and excellent reusability (89.1% over 20 cycles).
Conclusions:
- The developed microfluidic chip offers a reliable and efficient method for AFB1 detection.
- The chip's design enhances reaction rates and minimizes environmental interference.
- This technology is suitable for field detection, small molecule analysis, and high-throughput systems.
