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Updated: Nov 2, 2025
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
Published on: December 17, 2012
Extraction and characterization of cell wall polysaccharides from cranberry (Vaccinium macrocarpon var. Stevens)
Eugenio Spadoni Andreani1, Salwa Karboune1, Lan Liu1
1Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, 21111 Lakeshore, Sainte Anne de Bellevue, Quebec H9X 3V9, Canada.
Abstract:
Cranberries of Stevens variety, mainly used for juice production, were processed into pomace, from which alcohol insoluble solids (AIS) were obtained. The cell wall polysaccharides were sequentially extracted from AIS, and characterized in terms of monosaccharide profile, sugar linkage and molecular weight distribution. Pectic polysaccharides represented more than 90% of the carbohydrates contained in hot buffer (HA), chelating agents (CH) and diluted alkali (DA) extracts. HA extract contained homogalacturonan with 75% being methyl esterified, and pectic arabinan with traces of pectic galactan, type II arabinogalactan and 1,4-β-glucan. CH extract, recovered with the highest yield (11.0% w/w), was composed mainly of homogalacturonan. DA extract included homogalacturonan with 2% methyl esterification, abundant arabinan and galactans and traces of 1,4-β-glucan. Glucomannan, xylan and xyloglucan represented 66% of the carbohydrates present in the last concentrated alkali extract (CA), the rest being pectic arabinan and galactan. High molecular weight polysaccharides (>102 kDa) were identified in all extracts.
